pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
(Plasmid #155125)

• Purpose: Tol2 vector containing Cre-inducible QFGal4-switch
• Depositing Lab: Bret Pearson
• Publication: Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 10.1016/j.ydbio.2020.07.007.

Backbone

• Vector backbone: pT2-Ip2GI
• Backbone size w/o insert (bp): 7424
• Total vector size (bp): 10448
• Vector type: Cre/Lox ; Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: QFGal4-switch
• Species: S. cerevisiae (budding yeast), Synthetic; Neurospora crassa
• Insert Size (bp): 3024
• GenBank ID: 855828 3875756
• Promoter: Ubi

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: tgc ata caa ggt ttt atc ctg ct
• 3′ sequencing primer: GGA TAA CAA TTT CAC ACA GG

Resource Information

• Supplemental Documents:
  • pTol2_ubi_loxP_mScarletnls_SV40pA_loxP_QFGal4_SV40pA.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector backbone derived from ubi:zebrabow (Plasmid #118219). mScarletNLS was codon-optimized for zebrafish by Tod Thiele (UTSC). ubi:QFGal4-switch can be used for Cre-dependent QFGal4 expression in lineage tracing experiments