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pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
(Plasmid #155125)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 155125 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT2-Ip2GI
  • Backbone size w/o insert (bp) 7424
  • Total vector size (bp) 10448
  • Vector type
    Cre/Lox ; Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    QFGal4-switch
  • Species
    S. cerevisiae (budding yeast), Synthetic; Neurospora crassa
  • Insert Size (bp)
    3024
  • GenBank ID
    855828 3875756
  • Promoter Ubi

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer tgc ata caa ggt ttt atc ctg ct
  • 3′ sequencing primer GGA TAA CAA TTT CAC ACA GG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Vector backbone derived from ubi:zebrabow (Plasmid #118219). mScarletNLS was codon-optimized for zebrafish by Tod Thiele (UTSC). ubi:QFGal4-switch can be used for Cre-dependent QFGal4 expression in lineage tracing experiments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA was a gift from Bret Pearson (Addgene plasmid # 155125 ; http://n2t.net/addgene:155125 ; RRID:Addgene_155125)
  • For your References section:

    An optimized QF-binary expression system for use in zebrafish. Burgess J, Burrows JT, Sadhak R, Chiang S, Weiss A, D'Amata C, Molinaro AM, Zhu S, Long M, Hu C, Krause HM, Pearson BJ. Dev Biol. 2020 Jul 19. pii: S0012-1606(20)30202-5. doi: 10.1016/j.ydbio.2020.07.007. 10.1016/j.ydbio.2020.07.007 PubMed 32697972