pLENTI_hACE2_PURO
(Plasmid #155295)

• Purpose: Lentiviral vector to generate hACE2 stable expressing cell line
• Depositing Lab: Raffaele De Francesco
• Publication: De Francesco Lab Plasmids (unpublished)

Backbone

• Vector backbone: pLenti CMV
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable ; Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hACE2
• Species: H. sapiens (human)
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Promoter: CMV
• Tag / Fusion Protein:
  • C9 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pLENTI hACE2 PURO.gb
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry