pLENTI_hACE2_HygR
(Plasmid
#155296)
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PurposeLentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 155296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 * |
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Backbone
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Vector backbonepLenti CMV
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature30°C
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Growth Strain(s)NEB Stable ; Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehACE2
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SpeciesH. sapiens (human)
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter CMV
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Tag
/ Fusion Protein
- C9 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLENTI_hACE2_HygR was a gift from Raffaele De Francesco (Addgene plasmid # 155296 ; http://n2t.net/addgene:155296 ; RRID:Addgene_155296)