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pET15-UbE2W
(Plasmid #15809)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15809 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin conjugating enzyme UbE2W
  • Alt name
    UbE2W
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    489
  • GenBank ID
    NM_001001481
  • Entrez Gene
    UBE2W (a.k.a. UBC-16, UBC16)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-UbE2W was a gift from Wade Harper (Addgene plasmid # 15809 ; http://n2t.net/addgene:15809 ; RRID:Addgene_15809)
  • For your References section:

    Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin J, Li X, Gygi SP, Harper JW. Nature. 2007 Jun 28. 447(7148):1135-8. 10.1038/nature05902 PubMed 17597759