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pET21a(+)-Bst LF-6xHis
(Plasmid #159148)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 159148 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Bioscience
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 7137
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    large fragment of Geobacillus stearothermophilus DNA polymerase I
  • Alt name
    Bst LF
  • Species
    Geobacillus stearothermophilus
  • Insert Size (bp)
    1776
  • GenBank ID
    AAB52611.1
  • Tag / Fusion Protein
    • His (C terminal on insert)

Resource Information

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a(+)-Bst LF-6xHis was a gift from Andrea Pauli (Addgene plasmid # 159148 ; http://n2t.net/addgene:159148 ; RRID:Addgene_159148)
  • For your References section:

    A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner MJ, Ross JJ, Schnabl J, Dekens MPS, Heinen R, Grishkovskaya I, Bauer B, Stadlmann J, Menéndez-Arias L, Fritsche-Polanz R, Traugott M, Seitz T, Zoufaly A, Födinger M, Wenisch C, Zuber J, Pauli A, Brennecke J.. bioRxiv. 2020. 10.1101/2020.06.23.166397