pET21a(+)-HIV RT-6xHis-RBS-prot
(Plasmid #159149)

• Purpose: expresses His-tagged HIV reverse transcriptase and untagged HIV protease
• Depositing Lab: Andrea Pauli
• Publication: Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. doi: 10.3389/fmolb.2022.801309. eCollection 2022.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: EMD Bioscience
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 7600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
• Alt name: rev
• Alt name: HIV RT
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 1695
• Mutation: HIV-1 group M/subtype B – BH10 strain
• GenBank ID: D86068.1
• Tag / Fusion Protein:
  • His (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Human immunodeficiency virus 1 (HIV-1) protease
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 519

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The ORF from HIV-1 RT is based on the sequence of HIV-1 RT group M/subtype B (BH10 strain) obtained from Luis Menéndez-Arias (Spanish National Research Council, Madrid, Spain). The ORF from the HIV protease was obtained from Stuart Le Grice (National Cancer Institute, USA).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.06.23.166397v2 for bioRxiv preprint.