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pET21a(+)-HIV RT-6xHis-RBS-prot
(Plasmid #159149)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 159149 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Bioscience
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 7600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
  • Alt name
    rev
  • Alt name
    HIV RT
  • Species
    Human immunodeficiency virus 1
  • Insert Size (bp)
    1695
  • Mutation
    HIV-1 group M/subtype B – BH10 strain
  • GenBank ID
    D86068.1
  • Tag / Fusion Protein
    • His (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Human immunodeficiency virus 1 (HIV-1) protease
  • Species
    Human immunodeficiency virus 1
  • Insert Size (bp)
    519

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The ORF from HIV-1 RT is based on the sequence of HIV-1 RT group M/subtype B (BH10 strain) obtained from Luis Menéndez-Arias (Spanish National Research Council, Madrid, Spain). The ORF from the HIV protease was obtained from Stuart Le Grice (National Cancer Institute, USA).
  • Terms and Licenses
  • Industry Terms

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a(+)-HIV RT-6xHis-RBS-prot was a gift from Andrea Pauli (Addgene plasmid # 159149 ; http://n2t.net/addgene:159149 ; RRID:Addgene_159149)
  • For your References section:

    A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner MJ, Ross JJ, Schnabl J, Dekens MPS, Heinen R, Grishkovskaya I, Bauer B, Stadlmann J, Menéndez-Arias L, Fritsche-Polanz R, Traugott M, Seitz T, Zoufaly A, Födinger M, Wenisch C, Zuber J, Pauli A, Brennecke J.. bioRxiv. 2020. 10.1101/2020.06.23.166397