Green Fluorescent Protein
Fusion protein or tag:
C terminal on insert
The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-GFP is ~7407 bp.
( Search Vector Database)
Backbone size w/o insert (bp):
5' sequencing primer:
List of Sequencing Primers
3' sequencing primer:
Growth temperature (℃):
Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
High or low copy:
View sequences (1)
Terms and Licenses:
Ancillary Agreement for Plasmids Containing FP Materials
Addgene has sequenced a portion of this plasmid for verification.
here for the sequencing
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
15949" in your Materials and Methods section.