Addgene - pLOVE-Klf4 Plasmid Data

Plasmid 15950: pLOVE-Klf4

Gene/insert name:
Kruppel-like factor 4
Alt name:
Klf4, GKLF, EZF
Insert size:
2156
Species:
M. musculus (mouse)
Entrez Gene:
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Fusion protein or tag:
V5
Terminal:
C terminal on insert
Mutation:
The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Klf4 is ~8963 bp.
Vector backbone:
pLOVE
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8263
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
V5
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Miguel Ramalho-Santos
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch et al (Cell Stem Cell. 2007 Sep 13. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15950" in your Materials and Methods section.