pLOVE-Klf4
(Plasmid #15950)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLOVE
  • Backbone size w/o insert (bp) 8263
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Kruppel-like factor 4
  • Alt name
    Klf4, GKLF, EZF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2156
  • Mutation
    The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Klf4 is ~8963 bp.
  • Entrez Gene
    Klf4 (a.k.a. RP23-322L22.2, EZF, Gklf, Zie)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOVE-Klf4 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15950)
  • For your References section:

    Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358