1
Plasmid 15950: pLOVE-Klf4
  • Kruppel-like factor 4

  • Klf4, GKLF, EZF

  • 2156

  • M. musculus (mouse)

  • Klf4 (RP23-322L22.2, EZF, Gklf, Zie)

  • V5

  • C terminal on insert

  • The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Klf4 is ~8963 bp.

  • pLOVE
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 8263

  • n/a List of Sequencing Primers

  • V5

  • Ampicillin

  • Stbl3

  • 37

  • Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)

  • High Copy

  • View sequences (1)
  • Miguel Ramalho-Santos

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch et al (Cell Stem Cell. 2007 Sep 13. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15950" in your Materials and Methods section.