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p405GAL1
(Plasmid #15963)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15963 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS405
  • Backbone manufacturer
    Sikorski & Hieter, Genetics 122:19-27 (1989)
  • Backbone size w/o insert (bp) 3814
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p405GAL1
  • Alt name
    Galactokinase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2380
  • Mutation
    GAL1 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    GAL1 (a.k.a. YBR020W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglI (not destroyed)
  • 3′ cloning site BglI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was cut from Mumberg plasmid p415GAL1, which was obtained from ATCC.
  • Terms and Licenses

Depositor Comments

Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p405GAL1 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15963)