|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17437||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerSikorski & Hieter, Genetics 122:19-27 (1989)
- Backbone size w/o insert (bp) 2691
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGAL1 promoter + polylinker + CYC1 terminator
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2380
MutationGAL1 promoter is between SacI and XbaI restriction sites.
Entrez GeneGAL1 (a.k.a. YBR020W)
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site BglI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPlasmid was created by Nicolas Buchler. Insert was cut from Mumberg plasmid p416GAL1, which was obtained from ATCC.
Terms and Licenses
Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p406GAL1 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17437)