pBabe_Hyg_cOVA_T2A_mStrawberry
(Plasmid #161737)

• Purpose: Expression of cOVA and mStrawberry
• Depositing Lab: Alec Kimmelman
• Publication: Yamamoto et al Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. Epub 2020 Apr 22.

Backbone

• Vector backbone: pBabe-hygro
• Backbone manufacturer: Addgene #1765
• Total vector size (bp): 7132
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cytoplasmic ovalbumin
• Alt name: cOVA
• Species: G. gallus (chicken)
• Tag / Fusion Protein:
  • mStrawberry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cOVA sequence was subcloned from pCI-neo-cOVA (Addgene #25097).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note Addgene NGS found a few discrepancies in the gag (truncated) region but depositor has confirmed this does not affect plasmid function. Depositor has verified plasmid's capacity to over-express the expected gene in target cells.