pNeuLite PEA3 mt
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16248||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHer-2/neu promoter
SpeciesH. sapiens (human)
MutationPEA3 mt: PEA3 motif AGGAAG changed to AGCTCG
Entrez GeneERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
HER-2 promoter with PEA3 binding site mutated (see pNeuLite for wildtype information).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNeuLite PEA3 mt was a gift from Mien-Chie Hung (Addgene plasmid # 16248 ; http://n2t.net/addgene:16248 ; RRID:Addgene_16248)
For your References section:The ets protein PEA3 suppresses HER-2/neu overexpression and inhibits tumorigenesis. Xing X, Wang SC, Xia W, Zou Y, Shao R, Kwong KY, Yu Z, Zhang S, Miller S, Huang L, Hung MC. Nat Med. 2000 Feb . 6(2):189-95. 10.1038/72294 PubMed 10655108