Addgene - pLM-vexGFP-Oct4 Plasmid Data

Plasmid 22240: pLM-vexGFP-Oct4

Gene/insert name:
vexGFP_2A_OCT4
Alt name:
Oct4
Insert size:
1872
Species:
H. sapiens (human)
Species:
synthetic fluorescent protein
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Vector backbone:
N/A
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
6655
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Michel Sadelain
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Papapetrou et al (Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22240" in your Materials and Methods section.