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pLM-mCerulean-cMyc
(Plasmid #23244)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23244 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCerulean-2A-MYC
  • Alt name
    cMyc
  • Species
    H. sapiens (human); synthetic fluorescent protein
  • Insert Size (bp)
    2109
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequenced. These differences are not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLM-mCerulean-cMyc was a gift from Michel Sadelain (Addgene plasmid # 23244)
  • For your References section:

    Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64. 10.1073/pnas.0904825106 PubMed 19549847