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Depositing Lab
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Sequence Information
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Depositor Sequences: Full (1)
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Addgene Sequences: Partial (5)
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 23243 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 6655
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry_2A_KLF4
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Alt nameKLF4
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SpeciesH. sapiens (human); synthetic fluorescent protein
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Insert Size (bp)2193
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer hPGK-F (5'GTGTTCCGCATTCTGCAAG-3')
- 3′ sequencing primer WPRE-R (5'-CATAGCGTAAAAGGAGCAACA-3') (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLM-mCherry-Klf4 was a gift from Michel Sadelain (Addgene plasmid # 23243) -
For your References section:
Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64. 10.1073/pnas.0904825106 PubMed 19549847