Addgene - Tag5Amyc-GSK3b CA Plasmid Data

Plasmid 16261: Tag5Amyc-GSK3b CA

Gene/insert name:
GSK3 beta
Species:
H. sapiens (human)
Entrez Gene:
GSK3B ()
Fusion protein or tag:
Myc
Terminal:
C terminal on backbone
Mutation:
Constitutively active: S9A
Vector backbone:
pCMV-Tag 5A
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4300
Cloning site 5':
EcoRV
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Mien-Chie Hung
Terms and Licenses:
MTA

Comments:

S2A in the sequence does not affect function

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition. Zhou et al (Nat Cell Biol. 2004 Oct . 6(10):931-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16261" in your Materials and Methods section.