Addgene - pBS chick PEA3 [TJ#51] Plasmid Data

Plasmid 16279: pBS chick PEA3 [TJ#51]

Gene/insert name:
PEA3
Alt name:
Pea3
Insert size:
2500
Species:
G. gallus (chicken)
GenBank ID:
AF075708
Entrez Gene:
ETV4 (PEA3)
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Thomas Jessell
Terms and Licenses:
MTA

Comments:

To make an antisense probe, cut with NotI and transcribe with T7.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Functionally related motor neuron pool and muscle sensory afferent subtypes defined by coordinate ETS gene expression. Lin et al (Cell. 1998 Oct 30. 95(3):393-407. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16279" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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