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pMIR-R/Tac1/SG
(Plasmid #16296)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16296 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIR-Report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tac1 3'UTR
  • Alt name
    Tac1
  • Alt name
    PPT-1, PPT-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    534
  • GenBank ID
    NM_003182
  • Entrez Gene
    TAC1 (a.k.a. Hs.2563, NK2, NKNA, NPK, TAC2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer M13-F20
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-R/Tac1/SG was a gift from Pranela Rameshwar (Addgene plasmid # 16296 ; http://n2t.net/addgene:16296 ; RRID:Addgene_16296)
  • For your References section:

    MicroRNAs regulate synthesis of the neurotransmitter substance P in human mesenchymal stem cell-derived neuronal cells. Greco SJ, Rameshwar P. Proc Natl Acad Sci U S A. 2007 Sep 25. 104(39):15484-9. 10.1073/pnas.0703037104 PubMed 17855557