Addgene - Nanog5P reporter Plasmid Data

Gene/insert name:		Nanog promoter
Insert size (bp):		2500
Gene/insert aliases:		Nanog, ENK, ecat4, 2410002E02Rik
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		Luciferase
Terminal:		C terminal on backbone
Vector backbone:		pGL3-basic (Search Vector Database)
Backbone manufacturer:		Promega
Type of vector:		Mammalian expression,Luciferase
Backbone size (bp):		4800
Cloning site 5':		KpnI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		RVprimer3 (List of Sequencing Primers)
3' Sequencing primer:		LucNrev
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Austin Cooney
Terms and Licenses:		MTA

Comments: 5' Nanog Luciferase reporter, contains 2.5 kb of 5' promoter region of the mouse Nanog gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Gu P et al. (Mol Cell Biol. 2005 Oct . 25(19):8507-19. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 16337" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.