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pGEM-Oct4
(Plasmid #16352)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
  • Alt name
    POU5F1
  • Alt name
    Oct4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • GenBank ID
    NM_002701
  • Entrez Gene
    POU5F1 (a.k.a. DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

This plasmid contains the Oct4 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-Oct4 was a gift from James Thomson (Addgene plasmid # 16352)
  • For your References section:

    Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Nov 20. ():. 10.1126/science.1151526 PubMed 18029452