Addgene - pGEM-Oct4 Plasmid Data

Plasmid 16352: pGEM-Oct4

Gene/insert name:
Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
Alt name:
POU5F1
Alt name:
Oct4
Insert size:
1100
Species:
H. sapiens (human)
GenBank ID:
NM_002701
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Vector backbone:
pGEM-T Easy
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3000
5' sequencing primer:
M13 Reverse List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
James Thomson
Terms and Licenses:
MTA

Comments:

This plasmid contains the Oct4 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu et al (Science. 2007 Nov 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16352" in your Materials and Methods section.