|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16352||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameHomo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
SpeciesH. sapiens (human)
Insert Size (bp)1100
Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid contains the Oct4 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM-Oct4 was a gift from James Thomson (Addgene plasmid # 16352 ; http://n2t.net/addgene:16352 ; RRID:Addgene_16352)
For your References section:Induced pluripotent stem cell lines derived from human somatic cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Dec 21. 318(5858):1917-20. 10.1126/science.1151526 PubMed 18029452