1
Plasmid 16577: pSin-EF2-Sox2-Pur
  • Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)

  • Sox2

  • 1000

  • H. sapiens (human)

  • NM_003106

  • SOX2 (ANOP3, MCOPS3)

  • pSin4-EF2-IRES-Pur
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7500

  • EcoRI

  • No

  • SpeI

  • No

  • EF-1a Forward List of Sequencing Primers

  • n/a

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.

  • View sequences (2)
  • View map

  • Notes from Addgene (1)

  • James Thomson

  • MTA

Comments: 

The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu et al (Science. 2007 Nov 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16577" in your Materials and Methods section.