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Plasmid 16579: pSin-EF2-Oct4-Pur
  • Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1)

  • POU5F1

  • Oct4

  • 1100

  • H. sapiens (human)

  • NM_002701

  • POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

  • pSin4-EF2-IRES-Pur
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7500

  • SpeI

  • No

  • EcoRI

  • No

  • EF-1a Forward List of Sequencing Primers

  • n/a

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.

  • View sequences (2)
  • View map

  • James Thomson

  • UBMTA

Comments: 

The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu et al (Science. 2007 Nov 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16579" in your Materials and Methods section.