Addgene - pSin-EF2-Oct4-Pur Plasmid Data

Plasmid 16579: pSin-EF2-Oct4-Pur

Gene/insert name:
Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
Alt name:
POU5F1
Alt name:
Oct4
Insert size:
1100
Species:
H. sapiens (human)
GenBank ID:
NM_002701
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Vector backbone:
pSin4-EF2-IRES-Pur
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7500
Cloning site 5':
SpeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
EF-1a Forward List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
James Thomson
Terms and Licenses:
MTA

Comments:

The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu et al (Science. 2007 Nov 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16579" in your Materials and Methods section.