|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16579||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHomo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
SpeciesH. sapiens (human)
Insert Size (bp)1100
Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EF-1a Forward
- 3′ sequencing primer n/a (Common Sequencing Primers)
The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSin-EF2-Oct4-Pur was a gift from James Thomson (Addgene plasmid # 16579 ; http://n2t.net/addgene:16579 ; RRID:Addgene_16579)
For your References section:Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Nov 20. ():. 10.1126/science.1151526 PubMed 18029452