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Plasmid 16580: pSin-EF2-LIN28-Pur
  • Homo sapiens lin-28 homolog (C. elegans)

  • LIN28

  • 600

  • H. sapiens (human)

  • NM_024674

  • LIN28A (CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

  • pSin4-EF2-IRES-Pur
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7500

  • EcoRI

  • No

  • SpeI

  • No

  • EF-1a Forward List of Sequencing Primers

  • n/a

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.

  • View sequences (3)
  • View map

  • James Thomson

  • UBMTA

Comments: 

The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu et al (Science. 2007 Nov 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16580" in your Materials and Methods section.