pSin-EF2-LIN28-Pur
(Plasmid #16580)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pSin4-EF2-IRES-Pur
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Homo sapiens lin-28 homolog (C. elegans)
  • Alt name
    LIN28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • GenBank ID
    NM_024674
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.
  • Terms and Licenses

Depositor Comments

The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSin-EF2-LIN28-Pur was a gift from James Thomson (Addgene plasmid # 16580)
  • For your References section:

    Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Nov 20. ():. 10.1126/science.1151526 PubMed 18029452