- Backbone size w/o insert (bp) 7500
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHomo sapiens lin-28 homolog (C. elegans)
SpeciesH. sapiens (human)
Insert Size (bp)600
Entrez GeneLIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer EF-1a Forward
- 3′ sequencing primer n/a (Common Sequencing Primers)
The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSin-EF2-LIN28-Pur was a gift from James Thomson (Addgene plasmid # 16580)
For your References section:Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Nov 20. ():. 10.1126/science.1151526 PubMed 18029452
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.