pCEP9 MLH1
(Plasmid #16458)

• Depositing Lab: Bert Vogelstein
• Publication: Papadopoulos et al Science. 1994 Mar 18. 263(5153):1625-9.

Backbone

• Vector backbone: pCEP9
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MLH1
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: MLH1 (a.k.a. COCA2, FCC2, HNPCC, HNPCC2, LYNCH2, MLH-1, MMRCS1, hMLH1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBVrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry