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SARS-CoV-2 Spike-d18
(Plasmid #164583)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164583 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRP
  • Backbone manufacturer
    Vectorbuilder
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Spike
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    3768
  • Mutation
    Deleted amino acids 1256-1273
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter EF1a

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SinoBiological Cat No. VG40589-UT
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Codon-optimized SARS-CoV-2 Spike was cloned from SinoBiological plasmid VG40589-UT. An 18-amino acid truncation was introduced to improve expression. The plasmid can be used to generate Spike-pseudotyped lentiviral or VSV particles.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 Spike-d18 was a gift from Jason Sheltzer (Addgene plasmid # 164583 ; http://n2t.net/addgene:164583 ; RRID:Addgene_164583)