Addgene - pCMV-Neo-Bam APC 1-331 Plasmid Data

Plasmid 16508: pCMV-Neo-Bam APC 1-331

Gene/insert name:
APC
Insert size:
9000
Species:
H. sapiens (human)
Entrez Gene:
APC (BTPS2, DP2, DP2.5, DP3, GS, PPP1R46)
Mutation:
Codon 331 truncation
Vector backbone:
pCMV-Neo-Bam
(Search Vector Database)
Backbone manufacturer:
Vogelstein Lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6600
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

This truncation was created using site-directed mutagenesis.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin et al (Science. 1997 Mar 21. 275(5307):1787-90. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16508" in your Materials and Methods section.