mNP-Luc (PPARdelta promoter)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16532||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePPAR delta promoter NP
Alt namePPAR delta
SpeciesH. sapiens (human)
Insert Size (bp)800
MutationMutant binding sites
Entrez GenePPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site PvuII (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
mNP represents fragment NP with both potential Tcf-4-binding sites mutated. TRE1 and TRE2 contain four repeats of the two Tcf-4-binding sites, respectively. mTRE1 and mTRE2 are mutant forms of TRE1 and TRE2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mNP-Luc (PPARdelta promoter) was a gift from Bert Vogelstein (Addgene plasmid # 16532 ; http://n2t.net/addgene:16532 ; RRID:Addgene_16532)
For your References section:PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149
Map uploaded by the depositor.