|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16593||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep53 binding sites
SpeciesH. sapiens (human)
Insert Size (bp)100
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (destroyed during cloning)
- 3′ cloning site NheI (destroyed during cloning)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
The following oligo pairs containing two copies of wild-type p53 binding sites were used: 5'-CTAGGCTGCA AGTCCTGACT TGTCCACACT CTGCAAGTCC TGACTTGTCC-3' and 5'-CTAGGGACAA GTCAGGACTT GCAGAGTGTG GACAAGTCAG GACTTGCAGC-3'. The annealed oligonucleotide pairs were concatamerized and cloned into the NheI site of pBVLuc.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:4XBS2WT-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16593 ; http://n2t.net/addgene:16593 ; RRID:Addgene_16593)
For your References section:PUMA induces the rapid apoptosis of colorectal cancer cells. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. Mol Cell. 2001 Mar . 7(3):673-82. 10.1016/S1097-2765(01)00213-1 PubMed 11463391
Map uploaded by the depositor.