c-myc promoter (TBE 1/2 - wt)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16595||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-myc promoter (TBE 1/2 wt)
SpeciesH. sapiens (human)
Insert Size (bp)600
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
To construct pTBE1/2 plasmid, we used polymerase chain reaction (PCR) primers (5'-CTAGCTAGCCTAGCACCTTTGATTTCTCCC-3' and 5'-CGTGATATCCGCTTTGATCAAGAGTCCCAG-3') to amplify nt -576 to -1162 of the c-MYC promoter region. The PCR product was cloned into pBV-Luc.
TBE1 (Tcf-4 binding elements) wt sequence cctttgatt; TBE2 wt sequence gctttgatc.
See Author's Map and article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:c-myc promoter (TBE 1/2 - wt) was a gift from Bert Vogelstein (Addgene plasmid # 16595 ; http://n2t.net/addgene:16595 ; RRID:Addgene_16595)
For your References section:Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977
Map uploaded by the depositor.