c-myc promoter (TBE 1m/2m - mut)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16596||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namec-myc promoter (TBE 1m/2m mut)
SpeciesH. sapiens (human)
Insert Size (bp)600
MutationTcf-4 binding elements (TBEs) mutated
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
To construct pTBE1m/2m, we used a mutated TBE1 primer (5'-CTAGCTAGCACTGGTGCATCTCCCAAACCCGGCAGCCCG-3') and a mutated TBE2 primer (5'-CTGGATATCACTGGTGCATCCCAGGGAGAGTGGAGGAAAG-3'), and subcloned the products into pBV-Luc.
See Author's Map and article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:c-myc promoter (TBE 1m/2m - mut) was a gift from Bert Vogelstein (Addgene plasmid # 16596 ; http://n2t.net/addgene:16596 ; RRID:Addgene_16596)
For your References section:Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977
Map uploaded by the depositor.