Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24 - January 1, 2020. For more information, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pTBRII / CEP-Zeo / Hygro
(Plasmid #16622)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16622 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    pCMV-Zeo cassette inserted into pCEP4
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TGF beta type II receptor
  • Alt name
    TGFbRII
  • Alt name
    transforming growth factor, beta receptor II
  • Species
    H. sapiens (human)
  • Entrez Gene
    TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pGEX3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TGFbRII cDNA generously provided by Dr. Harvey Lodish and Dr. Robert Weinberg
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

An expression construct for the TGF-beta RII gene was constructed by placing a TGF-beta RII cDNA between a cytomegalovirus (CMV) promoter and an simian virus 40 polyadenylation signal.

Please note that according to the map, this plasmid has 2 CMV promoters, so sequence confirmation with this primer may have limited success. Restriction digest with BamHI/HindIII is recommended for plasmid confirmation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTBRII / CEP-Zeo / Hygro was a gift from Bert Vogelstein (Addgene plasmid # 16622 ; http://n2t.net/addgene:16622 ; RRID:Addgene_16622)
  • For your References section:

    Targeted deletion of Smad4 shows it is required for transforming growth factor beta and activin signaling in colorectal cancer cells. Zhou S, Buckhaults P, Zawel L, Bunz F, Riggins G, Dai JL, Kern SE, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2412-6. 10.1073/pnas.95.5.2412 PubMed 9482899