M11 Cardiac Actin Promoter pCarA
(Plasmid
#17148)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 17148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCSKA
- Backbone size w/o insert (bp) 4000
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Vector typeXenopus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecardiac actin promoter
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SpeciesX. laevis (frog)
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Insert Size (bp)3270
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GenBank IDX04669
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
Depositor Comments
Plasmid History:
Constructed by Enrique Amaya.
Note for Use:
Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M11 Cardiac Actin Promoter pCarA was a gift from Randall Moon (Addgene plasmid # 17148 ; http://n2t.net/addgene:17148 ; RRID:Addgene_17148)
Map uploaded by the depositor.
