Addgene - M11 Cardiac Actin Promoter pCarA Plasmid Data

Plasmid 17148: M11 Cardiac Actin Promoter pCarA

Gene/insert name:
cardiac actin promoter
Insert size:
3270
Species:
X. laevis (frog)
GenBank ID:
X04669
Vector backbone:
pCSKA
(Search Vector Database)
Vector type:
Xenopus expression
Backbone size w/o insert (bp):
4000
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

Plasmid History:
Constructed by Enrique Amaya.

Note for Use:
Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17148" in your Materials and Methods section.