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M20 siamois-luciferase p01234
(Plasmid #17157)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17157 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4818
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    siamois promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
  • Entrez Gene
    sia1.L (a.k.a. XELAEV_18019203mg, Xsia, sia, sia1, siamois, siamois-A)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Plasmid History:
Constructed by David Kimelman, 6/6/96 XVII p. 98.

Notes for Use:
The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating.

The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate.

Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M20 siamois-luciferase p01234 was a gift from Randall Moon (Addgene plasmid # 17157 ; ; RRID:Addgene_17157)