M20 siamois-luciferase p01234
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17157||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Gene/Insert namesiamois promoter
SpeciesX. laevis (frog)
Insert Size (bp)800
Entrez Genesia1.L (a.k.a. XELAEV_18019203mg, Xsia, sia, sia1, siamois, siamois-A)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Constructed by David Kimelman, 6/6/96 XVII p. 98.
Notes for Use:
The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating.
The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate.
Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M20 siamois-luciferase p01234 was a gift from Randall Moon (Addgene plasmid # 17157 ; http://n2t.net/addgene:17157 ; RRID:Addgene_17157)
Map uploaded by the depositor.