Addgene - M20 siamois-luciferase p01234 Plasmid Data

Plasmid 17157: M20 siamois-luciferase p01234

Gene/insert name:
siamois promoter
Insert size:
800
Species:
X. laevis (frog)
Entrez Gene:
sia1 (Xsia, siamois, siamois-A)
Fusion protein or tag:
luciferase
Terminal:
C terminal on backbone
Vector backbone:
pGL3-Basic
(Search Vector Database)
Backbone manufacturer:
promega
Vector type:
Luciferase
Backbone size w/o insert (bp):
4818
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
LucNRev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Plasmid History:
Constructed by David Kimelman, 6/6/96 XVII p. 98.

Notes for Use:
The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating.

The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate.

Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17157" in your Materials and Methods section.