Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7390
Vector typeYeast Expression
Selectable markersGeneticin (G418)
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- B42•HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CCAGCCTCTTGCTGAGTGGAGATG (Common Sequencing Primers)
Terms and Licenses
ref for pJG4-5: Gyuris, J., Golemis, E., Chertkov, H. & Brent, R. (1993) Cell 75, 791-803.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2GB42/Kan was a gift from Stuart Schreiber (Addgene plasmid # 17260 ; http://n2t.net/addgene:17260 ; RRID:Addgene_17260)
For your References section:A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. Huang J, Schreiber SL. Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401. 10.1073/pnas.94.25.13396 PubMed 9391035
Map uploaded by the depositor.