Addgene - p2GB42/Kan Plasmid Data

Plasmid 17260: p2GB42/Kan

Gene/insert name:
None
Fusion protein or tag:
B42•HA
Terminal:
N terminal on backbone
Vector backbone:
pJG4-5
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size (bp):
7390
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CCAGCCTCTTGCTGAGTGGAGATG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
Geneticin (G418)
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Stuart Schreiber
Terms and Licenses:
MTA

Comments:

ref for pJG4-5: Gyuris, J., Golemis, E., Chertkov, H. & Brent, R. (1993) Cell 75, 791-803.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A yeast genetic system for selecting small molecule inhibitors of protein-protein interactions in nanodroplets. Huang et al (Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17260" in your Materials and Methods section.