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pLexA
(Plasmid #11342)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11342 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLexA
  • Backbone manufacturer
    see "author's map". Not the same as Clontech's pLexA.
  • Backbone size (bp) 5700
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • LexA DNA Binding Domain (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Yeast two-hybrid DNA-binding domain vector, containing the MCS illustrated in the author's map. Use for traditional cloning for a target cDNA sequence for a yeast two-hybrid screen.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLexA was a gift from Guy Caldwell (Addgene plasmid # 11342)