Plasmid 11343: pACT2.2
  • None

  • Gal4 Activation Domain

  • N terminal on backbone

  • pACT2.2
    (Search Vector Database)

  • Yeast Expression

  • 7550

  • Gal4 AD List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • LEU2

  • View sequences (1)
  • View map

  • Guy Caldwell



Yeast two-hybrid transcriptional activation domain vector, containing the MCS illustrated in "author's map". Use for traditional cloning for a target cDNA sequence to test a directed yeast two-hybrid interaction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11343" in your Materials and Methods section.