- Backbone size (bp) 7550
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
/ Fusion Protein
- Gal4 Activation Domain (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer Gal4 AD (Common Sequencing Primers)
Yeast two-hybrid transcriptional activation domain vector, containing the MCS illustrated in "author's map". Use for traditional cloning for a target cDNA sequence to test a directed yeast two-hybrid interaction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pACT2.2 was a gift from Guy Caldwell (Addgene plasmid # 11343)
Map uploaded by the depositor.