Plasmid 11343: pACT2.2
  • None

  • Gal4 Activation Domain

  • N terminal on backbone

  • pACT2.2
    (Search Vector Database)

  • Yeast Expression

  • 7550

  • Gal4 AD List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • LEU2

  • View sequences (1)
  • View map

  • Guy Caldwell



Yeast two-hybrid transcriptional activation domain vector, containing the MCS illustrated in "author's map". Use for traditional cloning for a target cDNA sequence to test a directed yeast two-hybrid interaction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11343" in your Materials and Methods section.