Addgene - pACT2.2 Plasmid Data

Plasmid 11343: pACT2.2

Gene/insert name:
None
Fusion protein or tag:
Gal4 Activation Domain
Terminal:
N terminal on backbone
Vector backbone:
pACT2.2
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size (bp):
7550
5' sequencing primer:
Gal4 AD List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
LEU2
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Guy Caldwell
Terms and Licenses:
MTA

Comments:

Yeast two-hybrid transcriptional activation domain vector, containing the MCS illustrated in "author's map". Use for traditional cloning for a target cDNA sequence to test a directed yeast two-hybrid interaction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11343" in your Materials and Methods section.