Addgene - pAct::Raf Plasmid Data

Plasmid 11341: pAct::Raf

Gene/insert name:
byr-2
Alt name:
byr2
Alt name:
Raf homolog
Species:
S. pombe (fission yeast)
GenBank ID:
M74293
Entrez Gene:
byr2 ()
Fusion protein or tag:
Gal4 activation domain
Terminal:
N terminal on backbone
Vector backbone:
pGADGH
(Search Vector Database)
Vector type:
Yeast Expression
5' sequencing primer:
Gal4 AD List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
LEU2
Sequence: View sequences (1)
Principal Investigator:
Michael Wigler
Terms and Licenses:
MTA

Comments:

cDNA of fission yeast S. pombe byr-2 gene (Raf oncogene homolog) cloned into the yeast two-hybrid activation-domain vector (also called, pGADGH-byr2, Van Aelst et al., 1993, PNAS, 90(13):6213-7). pGADGH is a derivative of pGAD, which was described by Stanley Fields in PNAS Vol 88, p. 9578-9582. pGADGH contains the ADH1 promoter expressing GAL4 transcriptional activiation domain (aa768-881). The multiple cloning site of pGAD1 is replaced with the SpeI-KpnI fragment of the multiple cloning site of plasmid pBluescript SK (-).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Complex formation between RAS and RAF and other protein kinases. Van et al (Proc Natl Acad Sci U S A. 1993 Jul 1. 90(13):6213-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11341" in your Materials and Methods section.