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pAct::Raf
(Plasmid #11341)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 11341 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    byr-2
  • Alt name
    byr2
  • Alt name
    Raf homolog
  • Species
    S. pombe (fission yeast)
  • GenBank ID
    M74293
  • Entrez Gene
    byr2
  • Tag / Fusion Protein
    • Gal4 activation domain (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

cDNA of fission yeast S. pombe byr-2 gene (Raf oncogene homolog) cloned into the yeast two-hybrid activation-domain vector (also called, pGADGH-byr2, Van Aelst et al., 1993, PNAS, 90(13):6213-7). pGADGH is a derivative of pGAD, which was described by Stanley Fields in PNAS Vol 88, p. 9578-9582. pGADGH contains the ADH1 promoter expressing GAL4 transcriptional activiation domain (aa768-881). The multiple cloning site of pGAD1 is replaced with the SpeI-KpnI fragment of the multiple cloning site of plasmid pBluescript SK (-).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct::Raf was a gift from Michael Wigler (Addgene plasmid # 11341 ; http://n2t.net/addgene:11341 ; RRID:Addgene_11341)
  • For your References section:

    Complex formation between RAS and RAF and other protein kinases. Van Aelst L, Barr M, Marcus S, Polverino A, Wigler M. Proc Natl Acad Sci U S A. 1993 Jul 1. 90(13):6213-7. 10.1073/pnas.90.13.6213 PubMed 8327501