Addgene - pLexA::Ras Plasmid Data

Plasmid 11340: pLexA::Ras

Gene/insert name:
Ras1
Alt name:
Ras-1
Insert size:
660
Species:
S. pombe (fission yeast)
Entrez Gene:
ras1 ()
Fusion protein or tag:
LexA DNA binding domain
Terminal:
N terminal on backbone
Vector backbone:
pLexA
(Search Vector Database)
Backbone manufacturer:
see pLexA map in "author's map"
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5700
5' sequencing primer:
LexA sequencing primer (CGTCAGCAGAGCTTCACCATTG) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
TRP1
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Michael Wigler
Terms and Licenses:
MTA

Comments:

cDNA of fission yeast S. pombe Ras1 gene (oncogene) cloned into the yeast two-hybrid DNA-binding domain vector pLexA (ref., Chang et al., 2004, Cell, 79:131-141). See "author's map" for picture of empty pLexA vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Chang et al (Cell. 1994 Oct 7. 79(1):131-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11340" in your Materials and Methods section.