|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11340||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturersee pLexA map in "author's map"
- Backbone size w/o insert (bp) 5700
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. pombe (fission yeast)
Insert Size (bp)660
Entrez Generas1 (a.k.a. SPAC17H9.09c, ste5)
/ Fusion Protein
- LexA DNA binding domain (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer LexA sequencing primer (CGTCAGCAGAGCTTCACCATTG) (Common Sequencing Primers)
Terms and Licenses
cDNA of fission yeast S. pombe Ras1 gene (oncogene) cloned into the yeast two-hybrid DNA-binding domain vector pLexA (ref., Chang et al., 2004, Cell, 79:131-141). See "author's map" for picture of empty pLexA vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLexA::Ras was a gift from Michael Wigler (Addgene plasmid # 11340 ; http://n2t.net/addgene:11340 ; RRID:Addgene_11340)
For your References section:Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Chang EC, Barr M, Wang Y, Jung V, Xu HP, Wigler MH. Cell. 1994 Oct 7. 79(1):131-41. 10.1016/0092-8674(94)90406-5 PubMed 7923372
Map uploaded by the depositor.