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pLexA::Ras
(Plasmid #11340)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11340 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLexA
  • Backbone manufacturer
    see pLexA map in "author's map"
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ras1
  • Alt name
    Ras-1
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    660
  • Entrez Gene
    ras1 (a.k.a. SPAC17H9.09c, ste5)
  • Tag / Fusion Protein
    • LexA DNA binding domain (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

cDNA of fission yeast S. pombe Ras1 gene (oncogene) cloned into the yeast two-hybrid DNA-binding domain vector pLexA (ref., Chang et al., 2004, Cell, 79:131-141). See "author's map" for picture of empty pLexA vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLexA::Ras was a gift from Michael Wigler (Addgene plasmid # 11340 ; http://n2t.net/addgene:11340 ; RRID:Addgene_11340)
  • For your References section:

    Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Chang EC, Barr M, Wang Y, Jung V, Xu HP, Wigler MH. Cell. 1994 Oct 7. 79(1):131-41. 10.1016/0092-8674(94)90406-5 PubMed 7923372