Addgene - pACT2.2gtwy Plasmid Data

Plasmid 11346: pACT2.2gtwy

Gene/insert name:
gateway cassette
Fusion protein or tag:
Gal4 Activation Domain
Terminal:
N terminal on backbone
Vector backbone:
pACT2.2
(Search Vector Database)
Backbone manufacturer:
see map of pACT2.2 in "author's map"
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
7550
Cloning method:
Gateway Cloning
Cloning site 5':
SmaI
Site destroyed during cloning:
Unknown
Cloning site 3':
SmaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Gal4 AD List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
This vector is propagated in E. coli strain DB3.1, due to the requirement to circumvent the lethality of the inherent ccdB gene until an insert is recombined into the Gateway cassette.
High or low copy:
High Copy
Selectable markers:
LEU2
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Guy Caldwell
Terms and Licenses:
MTA

Comments:

Gateway-modified yeast two-hybrid Activation domain vector. Use this vector for Gateway cloning of a cDNA to be used to test a directed yeast two-hybrid interaction. This vector was modified from plasmid pACT2.2 by placing a Gateway acceptor cassette in the SmaI site of pACT2.2. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.) Please note that the "author's map" is the map of the original pACT2.2 vector upon which pACT2.2gtwy is derived - it is not a map of pACT2.2gtwy.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11346" in your Materials and Methods section.