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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC8
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 2700
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Vector typefor maintenance
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namestreptavidin
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SpeciesStreptomyces avidinii
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Insert Size (bp)2000
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GenBank IDX03591
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13 forward20
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was cloned in Charles Cantor's lab in Columbia University
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A 2kb fragment shown in Fig 2A of PubMed 3951999 was cloned into pUC-8. The streptavidin gene is roughly 500 bp away from the M13-forward20 primer.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC8-SZ was a gift from Takeshi Sano (Addgene plasmid # 17330 ; http://n2t.net/addgene:17330 ; RRID:Addgene_17330) -
For your References section:
Molecular cloning and nucleotide sequence of the streptavidin gene. Argarana CE, Kuntz ID, Birken S, Axel R, Cantor CR. Nucleic Acids Res. 1986 Feb 25. 14(4):1871-82. 10.1093/nar/14.4.1871 PubMed 3951999
Map uploaded by the depositor.
