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pBabe zeo HMGA2
(Plasmid #17411)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBabe-Zeo
  • Backbone manufacturer
    Available at Addgene (#1766)
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HMGA2
  • Alt name
    high mobility group AT-hook 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    330
  • Entrez Gene
    Hmga2 (a.k.a. 9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pBabe 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HMGA2 was a kind gift of C. Mayr
  • Terms and Licenses

Depositor Comments

The ORF of human HMGA2 was amplified using HMGA2F (5’-TAAGGATCCATGAGCGCACGCGGTGAG-3’) and HMGA2R (5’- GCGGAATTCCTAATCCTCCTCTGCGGACTCTTG-3’). The product was cut with BamHI and EcoRI and cloned into pBabe.Zeo.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe zeo HMGA2 was a gift from Tyler Jacks (Addgene plasmid # 17411)
  • For your References section:

    Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks T. Proc Natl Acad Sci U S A. 2008 Feb 28. ():. 10.1073/pnas.0712321105 PubMed 18308936