Addgene - pBabe zeo HMGA2 Plasmid Data

Plasmid 17411: pBabe zeo HMGA2

Gene/insert name:
HMGA2
Alt name:
high mobility group AT-hook 2
Insert size:
330
Species:
M. musculus (mouse)
Entrez Gene:
Hmga2 (9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)
Vector backbone:
pBabe-Zeo
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#1766)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
4900
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
pBabe 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Person or lab that originally
cloned the gene/insert:
HMGA2 was a kind gift of C. Mayr
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

The ORF of human HMGA2 was amplified using HMGA2F (5’-TAAGGATCCATGAGCGCACGCGGTGAG-3’) and HMGA2R (5’- GCGGAATTCCTAATCCTCCTCTGCGGACTCTTG-3’). The product was cut with BamHI and EcoRI and cloned into pBabe.Zeo.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar et al (Proc Natl Acad Sci U S A. 2008 Feb 28. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17411" in your Materials and Methods section.