|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25404||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLentilox 3.7GFP
Backbone manufacturerUniversity of Michigan Vector Core
- Backbone size w/o insert (bp) 7650
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)85
Entrez GeneMirlet7b (a.k.a. Mirnlet7b, let-7b, mmu-let-7b)
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site Xho1 (destroyed during cloning)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The last nucleotide in the Letb insert is G rather than A but will not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Let-7b-GFP was a gift from Sean Morrison (Addgene plasmid # 25404 ; http://n2t.net/addgene:25404 ; RRID:Addgene_25404)
For your References section:Hmga2 promotes neural stem cell self-renewal in young but not old mice by reducing p16Ink4a and p19Arf Expression. Nishino J, Kim I, Chada K, Morrison SJ. Cell. 2008 Oct 17. 135(2):227-39. 10.1016/j.cell.2008.09.017 PubMed 18957199