Let-7b-GFP
(Plasmid #25404)

• Depositing Lab: Sean Morrison
• Publication: Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Backbone

• Vector backbone: pLentilox 3.7GFP
• Backbone manufacturer: University of Michigan Vector Core
• Backbone size w/o insert (bp): 7650
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mirlet7b
• Alt name: Let-7b
• Species: M. musculus (mouse)
• Insert Size (bp): 85
• Mutation: None
• GenBank ID: NM_029727
• Entrez Gene: Mirlet7b (a.k.a. Mirnlet7b, let-7b, mmu-let-7b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Xho1 (destroyed during cloning)
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The last nucleotide in the Letb insert is G rather than A but will not affect function.