|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14784||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN5' (Common Sequencing Primers)
Terms and Licenses
miRNA of the following mature sequence, with approximately 500 basepairs of flanking sequence, were cloned into MSCV-neo: let-7g: 5’-UGAGG UAGUA GUUUG UACAG U-3’.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV let-7g was a gift from Tyler Jacks (Addgene plasmid # 14784 ; http://n2t.net/addgene:14784 ; RRID:Addgene_14784)
For your References section:Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365