Addgene - MSCV let-7g Plasmid Data

Plasmid 14784: MSCV let-7g

Gene/insert name:
let-7g
Species:
M. musculus (mouse)
Vector backbone:
MSCV-neo
(Search Vector Database)
Backbone manufacturer:
BD Biosciences
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6500
5' sequencing primer:
pLXSN5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

miRNA of the following mature sequence, with approximately 500 basepairs of flanking sequence, were cloned into MSCV-neo: let-7g: 5’-UGAGG UAGUA GUUUG UACAG U-3’.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar et al (Nat Genet. 2007 Apr 1. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14784" in your Materials and Methods section.