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Plasmid 17410: Puro.let-7gsm.Cre
  • Let-7g mutant

  • Let7 g

  • 500

  • M. musculus (mouse)

  • mutated version of let-7g

  • Puro.Cre
    (Search Vector Database)

  • Jacks Lab

  • Mammalian Expression, Lentiviral, Cre/Lox

  • 9200

  • XhoI

  • No

  • NotI

  • Yes

  • n/a List of Sequencing Primers

  • MSCV rev

  • Ampicillin

  • Stbl3

  • 37

  • Unknown

  • Puromycin

  • View sequences (2)
  • View map

  • Tyler Jacks

  • MTA

Comments: 

Puro.let7gsm.Cre was generated by two rounds site-directed mutagenesis of Puro.let7g.Cre with mut1F (5’-GATTCCAGGCTGACCTAGTAGTTTGTACAGTTTGAGGGTCTATGATAC-3’), mut1R (5’-GTATCATAGACCCTCAAACTGTACAAACTACTAGGTCAGCCTGGAATC-3’), mut2F (5’-CAGGAGATAACTGTACAGGCCACTGGGTTGCCAGGAAC-3’), and mut2R (5’-GTTCCTGGCAACCCAGTGGCCTGTACAGTTATCTCCTG-3’).

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar et al (Proc Natl Acad Sci U S A. 2008 Feb 28. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17410" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only