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Plasmid 17409: Puro.let-7g.Cre
  • Let-7g

  • Let7 g

  • 500

  • M. musculus (mouse)

  • Puro.Cre
    (Search Vector Database)

  • Jacks Lab

  • Mammalian Expression, Lentiviral, Cre/Lox

  • 9200

  • XhoI

  • No

  • NotI

  • Yes

  • n/a List of Sequencing Primers

  • MSCV rev

  • Ampicillin

  • Stbl3

  • 37

  • Unknown

  • Puromycin

  • View sequences (2)
  • View map

  • Tyler Jacks

  • UBMTA

Comments: 

. Let-7g was amplified from murine genomic DNA with purolet7gF (5’-GTGGCGCTCGAGAATATATATAAATGACTGGTGTATTTC-3’) and purolet7gR (5’-TAGCGGCCGCGAACCCTTCTATATATTCTG-3’), digested with XhoI and NotI and cloned into Puro.Cre to generate Puro.let7g.Cre.

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar et al (Proc Natl Acad Sci U S A. 2008 Feb 28. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17409" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only