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Puro.let-7g.Cre
(Plasmid #17409)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    Puro.Cre
  • Backbone manufacturer
    Jacks Lab
  • Backbone size w/o insert (bp) 9200
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Let-7g
  • Alt name
    Let7 g
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer MSCV rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

. Let-7g was amplified from murine genomic DNA with purolet7gF (5’-GTGGCGCTCGAGAATATATATAAATGACTGGTGTATTTC-3’) and purolet7gR (5’-TAGCGGCCGCGAACCCTTCTATATATTCTG-3’), digested with XhoI and NotI and cloned into Puro.Cre to generate Puro.let7g.Cre.

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Puro.let-7g.Cre was a gift from Tyler Jacks (Addgene plasmid # 17409)
  • For your References section:

    Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks T. Proc Natl Acad Sci U S A. 2008 Feb 28. ():. 10.1073/pnas.0712321105 PubMed 18308936