Addgene - Puro.let-7g.Cre Plasmid Data

Plasmid 17409: Puro.let-7g.Cre

Gene/insert name:
Let-7g
Alt name:
Let7 g
Insert size:
500
Species:
M. musculus (mouse)
Vector backbone:
Puro.Cre
(Search Vector Database)
Backbone manufacturer:
Jacks Lab
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
9200
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
MSCV rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

. Let-7g was amplified from murine genomic DNA with purolet7gF (5’-GTGGCGCTCGAGAATATATATAAATGACTGGTGTATTTC-3’) and purolet7gR (5’-TAGCGGCCGCGAACCCTTCTATATATTCTG-3’), digested with XhoI and NotI and cloned into Puro.Cre to generate Puro.let7g.Cre.

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar et al (Proc Natl Acad Sci U S A. 2008 Feb 28. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17409" in your Materials and Methods section.