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Depositing Lab
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Publication
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Sequence Information
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Depositor Sequences: None.
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Addgene Sequences: Partial (1)
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 20931 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepsiCHECK-2
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 6273
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Vector typeMammalian Expression, Insect Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name8x let-7 target sites
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Insert Size (bp)216
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer TAAGTACATCAAGAGCTTCG (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Article Citing this Plasmid
Depositor Comments
Single let7 site sequence is - 5'-TCGAGACTATACAAGGATCTACCTCAG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psiCHECK2-let-7 8x was a gift from Yukihide Tomari (Addgene plasmid # 20931) -
For your References section:
Drosophila Argonaute1 and Argonaute2 Employ Distinct Mechanisms for Translational Repression. Iwasaki S, Kawamata T, Tomari Y. Mol Cell. 2009 Mar 4. ():. 10.1016/j.molcel.2009.02.010 PubMed 19268617