|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20932||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Vector typeMammalian Expression, Insect Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name8x let-7 target sites
Insert Size (bp)216
Mutation168bp IRES from 5'UTR of reaper gene in Drosophila was inserted in the 5'UTR of RL.
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer TAAGTACATCAAGAGCTTCG (Common Sequencing Primers)
Single let7 site sequence is - 5'-TCGAGACTATACAAGGATCTACCTCAG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCHECK2-IRES-let-7 8x was a gift from Yukihide Tomari (Addgene plasmid # 20932 ; http://n2t.net/addgene:20932 ; RRID:Addgene_20932)
For your References section:Drosophila Argonaute1 and Argonaute2 Employ Distinct Mechanisms for Translational Repression. Iwasaki S, Kawamata T, Tomari Y. Mol Cell. 2009 Mar 4. ():. 10.1016/j.molcel.2009.02.010 PubMed 19268617