Addgene - psiCHECK2-let-7 2x Plasmid Data

Plasmid 20929: psiCHECK2-let-7 2x

Gene/insert name:
2x let-7 target sites
Insert size:
54
Vector backbone:
psiCHECK-2
(Search Vector Database)
Vector type:
Mammalian Expression, Insect Expression, Luciferase
Backbone size w/o insert (bp):
6273
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
TAAGTACATCAAGAGCTTCG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Yukihide Tomari
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Single let7 site sequence is - 5'-TCGAGACTATACAAGGATCTACCTCAG

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Drosophila Argonaute1 and Argonaute2 Employ Distinct Mechanisms for Translational Repression. Iwasaki et al (Mol Cell. 2009 Mar 4. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20929" in your Materials and Methods section.