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pmiReport SHIP1 3' UTR
(Plasmid #26531)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pmiReport
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SHIP1 3'UTR
  • Alt name
    SHIP1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1179
  • Entrez Gene
    Inpp5d (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, p150Ship, s-SHIP)
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer M13pUC-fwd
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The inserts put into this vector are downstream from luciferase. They create different 3' UTRs for luciferase.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiReport SHIP1 3' UTR was a gift from David Baltimore (Addgene plasmid # 26531)
  • For your References section:

    Inositol phosphatase SHIP1 is a primary target of miR-155. O'Connell RM, Chaudhuri AA, Rao DS, Baltimore D. Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8. 10.1073/pnas.0902636106 PubMed 19359473