pmiReport SHIP1 3' UTR
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26531||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameSHIP1 3'UTR
SpeciesM. musculus (mouse)
Insert Size (bp)1179
Entrez GeneInpp5d (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, p150Ship, s-SHIP)
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
The inserts put into this vector are downstream from luciferase. They create different 3' UTRs for luciferase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmiReport SHIP1 3' UTR was a gift from David Baltimore (Addgene plasmid # 26531 ; http://n2t.net/addgene:26531 ; RRID:Addgene_26531)
For your References section:Inositol phosphatase SHIP1 is a primary target of miR-155. O'Connell RM, Chaudhuri AA, Rao DS, Baltimore D. Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8. 10.1073/pnas.0902636106 PubMed 19359473