Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26527||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepMSCV puro
- Backbone size w/o insert (bp) 6300
Growth in Bacteria
Alt nameBIC (miRNA containing region of Exon2)
SpeciesM. musculus (mouse)
Entrez GeneMir155 (a.k.a. Mirn155, mir-155, mmu-mir-155)
/ Fusion Protein
- GFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
pMGP is a modified MSCV vector whereby GFP was placed downstream from the 5′ LTR, and the miR-155 expression cassette was cloned downstream from the GFP stop codon.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MGP M155 was a gift from David Baltimore (Addgene plasmid # 26527 ; http://n2t.net/addgene:26527 ; RRID:Addgene_26527)
For your References section:Inositol phosphatase SHIP1 is a primary target of miR-155. O'Connell RM, Chaudhuri AA, Rao DS, Baltimore D. Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8. 10.1073/pnas.0902636106 PubMed 19359473